Evaluation of the Effects of Some Raphanus sativus Tuber Extracts on Certain Antioxidant Enzyme Activity and Lipid Peroxidation Level Against Gray Mold (Botrytis cinerea) in Vicia faba Leaves

The antioxidant defense system of the medicinal plant Raphanus sativus L. tuber root in terms certain enzymes (ascorbat peroxidase, glutathion reductase, catalase, superoxide dismutase) and lipid peroxidation against gray mold disease caused by Botrytis cinerea fungus on Vicia faba (bean) leaves was analyzed spectrophotometrically. V. plants were grown from seed under controlled conditions. A spore suspension 105 spores/ml B. prepared. treated with 1-) only distilled water, 2-)only DMSO, 3-) (positive control), 4-) extract applications (distilled ethanol methanol extract),5-) treatment after (extract:fungus). harvested 24 48 hours applications. Analyzes supernatants obtained extracts performed It has been revealed that activity all investigated generally increased extract:fungus application groups compared to group. determined alone an increase other than groups. observed there (MDA) group except 10mg/ml water treatment. As a result research, it enzyme levels decreased MDA content when negative control positive In applications, activities extract:fungus, EtOH MeOH From this point view, can be stated R. give response inoculation alone.